• 藍色版面
  • 綠色版面
  • 橘色版面
  • 粉紅色版面
  • 棕色版面
帳號:guest(120.119.126.29)          離開系統
字體大小: 字級放大   字級縮小   預設字形  

詳目顯示

研究生: 趙自屏
研究生(外文): chao tzu ping
論文名稱: CoQ10微脂粒粉末材料製備研究
論文名稱(外文): A Study on Preparation of Powder Liposomes Containing CoQ10
指導教授: 陳崇裕
指導教授(外文): chonyu  chen
學位類別: 碩士
校院名稱: 樹德科技大學
系所名稱: 應用設計研究所
論文出版年: 2008
畢業學年度: 97
語文別: 中文
論文頁數: 63
中文關鍵詞: 微脂粒Co Q10包覆率冷凍乾燥法
外文關鍵詞: liposomeCoQ10encapsulation efficiencyfreeze drying
相關次數:
  • 被引用:0
  • 點閱:36
  • 評分:*****
  • 下載:0
  • 書目收藏:0
本實驗研究利用微脂粒作為傳送載體的特性,應用微脂粒包覆Co Q10,再使用冷凍乾燥法進行樣本乾燥,使其變為粉末狀微脂粒再進行功能性評估。觀察其粒徑的大小分佈、安定性及最佳包覆效果,從而評估液狀及粉末狀微脂粒兩者之間的差別。
    本實驗是以酒精注射法來製備微脂粒,包覆抗老化的產品,也就是脂溶性抗氧化劑的Co Q10。實驗觀測的結果顯示,粉末狀的微脂粒在溫度4℃儲存35天後,粒徑在125 nm~688 nm,而液狀的微脂粒在溫度4℃儲存35天後,粒徑約在95 nm~459 nm。從微脂粒的包覆率上觀察,粉末狀微脂粒優於液狀的微脂粒,樣品乾燥前的包覆率約在80.56%~88.31%,乾燥後包覆率則在81.19%~92.22%之間;在表面電位上測得的微脂粒其在乾燥前,測得的電位在-17.6~-36.0 mV之間,而在樣品乾燥之後,電位則在-32.9~-63.5 mV之間。電位會隨著樣品乾燥後負電荷更大。針對在皮膚保濕度上的評估,則以添加膽固醇及只有包覆Co Q10的樣品組為最佳。
    人體實驗顯示,經24小時發現,Co Q10微脂粒並不會對皮膚造成刺激性及傷害性,其安全性極佳,應有商業運用價值。
This experimental study used liposomes to encapsulate CoQ10 based on the characteristics of liposomes as carriers, and then adopted the freeze drying method to dry the samples and prepare dry powder liposomes for an efficacy evaluation.  The particle size distribution, stability, and optimal encapsulation efficiency of liposomes were observed, and the difference between liquid liposomes and powder liposomes were evaluated.
  In this experiment, the liposomes were prepared using the ethanol injection method to encapsulate CoQ10, a lipid-soluble antioxidant that has anti-aging effects.  The observation results show that after both liquid liposomes and powder liposomes were stored at 4°C for 35 days, the particle size of powder liposomes was between 125nm and 688nm, whereas that of liquid liposomes was between 95.32nm and 459.8nm.  In terms of liposomal encapsulation efficiency, the encapsulation efficiency of powder liposomes was superior to that of liquid liposomes, since the encapsulation efficiency prior to drying of the samples was between 80.56% and 88.31%, and then ranged between 81.19% and 92.22% after drying.  The measured zeta potential of liposomes was -17.58~-36.03mV prior to drying, and -32.9~-63.5mV after drying.  The potential of the liposome samples increased along with negative charges after drying.  Regarding the evaluation of skin hydration, the CoQ10 liposomes with cholesterol added and cholesterol-free CoQ10 liposomes have the optimal effects.
  The human patch test shows that CoQ10 liposomes did not cause any irritation or damage to the skin after 24 hours, thus proving excellent safety and commercial value of CoQ10 liposomes.
中文摘要...................................................................................................................................Ⅰ
英文摘要...................................................................................................................................Ⅱ
誌謝..............................................................................................................................................Ⅲ
目錄..............................................................................................................................................Ⅳ
表目錄..........................................................................................................................................Ⅵ
圖目錄..........................................................................................................................................Ⅶ
第一章 緒論..............................................................................................................................1
1.1研究背景與動機.............................................................................................................1
1.2研究目的.........................................................................................................................4
1.3研究架構..........................................................................................................................6
第二章  文獻回顧.......................................................................................................................7
2.1微脂粒簡介.....................................................................................................................7
  2.1.1微脂粒的組成........................................................................................................8
  2.1.2微脂粒的分類........................................................................................................8
  2.1.3微脂粒的作用機制.............................................................................................10
2.2 Co Q10簡介..................................................................................................................12
2.3 Co Q10的功能.............................................................................................................12
2.4微脂粒的製備方式......................................................................................................13
2.5微脂粒的穩定性...........................................................................................................16
2.6微脂粒與皮膚的作用機制..........................................................................................19
2.6.1微脂粒與皮膚穿透能力之關係.......................................................................20
2.6.2微脂粒對皮膚的作用........................................................................................21
    2.7 微脂粒之粒徑分佈指數............................................................................................21
2.8 冷凍乾燥機的原理.....................................................................................................21
2.8.1冷凍乾燥法的步驟..........................................................................................22
2.8.2冷凍乾燥的優點...............................................................................................24
2.8.3冷凍乾燥的缺點...............................................................................................25
第三章 材料和方法...................................................................................................................27
3.1 藥品..............................................................................................................................27
3.2儀器及器材.................................................................................................................27
3.3實驗配方.......................................................................................................................28
3.4微脂粒製備實驗方法..................................................................................................29
3.4.1實驗流程............................................................................................................29
    3.5實驗方法與步驟...........................................................................................................30
3.5.1磷酸緩衝溶液的調配........................................................................................30
3.5.2粉末狀微脂粒製備方法...................................................................................30
3.5.3 粉末狀與液狀微脂粒粒徑的分佈檢測..........................................................31
3.5.4微脂粒之表面電位測定...................................................................................32
3.5.5 Co Q10濃度檢量線..........................................................................................33
3.5.6微脂粒之包覆率檢測........................................................................................34
3.5.7微脂粒安定性的測定........................................................................................34
3.5.8 皮膚保溼效果測定............................................................................................35
3.5.9皮膚過敏測試....................................................................................................36
第四章 結果與討論...................................................................................................................38
4.1 Co Q1濃度檢量線.......................................................................................................38
4.2 Co Q10粉末狀微脂粒水份逸失............................................................................... 39
4.3膽固醇與磷脂質間不同配方對粒徑與表面電位之影響.......................................40
4.4酒精注射法儲存於不同時間下粒徑的變化............................................................41
4.5微脂粒包覆率之測定................................................................................................. 46
4.6微脂粒對電位之測量................................................................................................. 48
4.7 Co Q10微脂粒之人體臨床試驗其安全性...............................................................51
4.8 Co Q10微脂粒對皮膚保濕效果之測定.................................................................. 52
第五章 結論.............................................................................................................................. 54
5.1結論................................................................................................................................54
5.2建議................................................................................................................................55
參考文獻......................................................................................................................................56
附    錄......................................................................................................................................62
英文部分
1.Bangham, A. D., Standish, M. M. and Watkins, J. C., 1965 , “Diffusion of Unrelevant Ions Across the Lamellae of Swollen Phospholipids”, Journal of Molecular Biology , vol. 13 , pp. 238-252.
2.Batzri, S. and Korn, E. D., 1973, “Single bilayer liposomes prepared without sonication”, Biochimica et Biophysica Acta , vol. 298 , pp.1015-1019.
3.Bonetti, A., Solito, F., Carmosino, G., Bargossi, A. M. and Fiorella, P. L., 2000 ,“Effect of ubidecarenone oral treatment on aerobic power in middle-aged trained subjects”, The Journal of Sports Medicine and Physical Fitness, vol.40 , pp. 51-57.
4.Barel, A. O. and Clarys, P. , 1995. “Measurement of epidermal capacitance”.
5.Crane, F. L., Hatefi, Y., Lester, R. I. and Widmer, C., 1957 ,“Isolation of a quinone from beef heart mitochondria”, Biochimica et Biophysica Acta , vol. 25 , pp. 220-221.
6.Draize, F., Woodard, G .and Calvery, H. O., 1948. “Appraisel of the safety of chemicals in food, drugs and cosmetics”, Pharmacologic , vol. 93 , pp. 377-392.
7.Deamer, D. W. and Bangham, A. D., 1976 ,“Large volume liposomes by an ether vaporization method”, Biochimica et Biophysica Acta , vol. 443 , pp. 629-634.
8.Deamer, D. W., 1978,“Preparation and properties of ether–injection liposomes”, Annals of the New York Academy of Sciences , vol. 308 , pp. 250-258.
9.Du Plessis, J., Ramachandran, C., Weiner, N. and Muller D. G., 1994,“The influence of particle size of liposomes on the disposition of drug into the skin”, International Journal of Pharmaceutics , vol. 103 , pp. 277-282.
10.Dreher, F., Luoso, P. L., Walde, P. and Elsner, P., 1996 ,“Human skin irritation studies of a lecithin microemulsion gel and of lecithin liposomes”, Skin Pharmacology and Applied Skin Physiology , vol. 9 , pp. 124-129.
11.Edgar, M. F., Michel, C. O. and Purmann. T. A., 1996,“Stable small particle liposome preparations, their production and use in topical cosmetic, and pharmaceutical compositions”, United States Patent , vol. 5 , pp. 498,420.
12.Fraley, R., Subramani, S., Berg, P. and Papahadjopoulos, D., 1980,“Introduction of Liposome-Encapsulated SV40 DNA into Cells”, Journal of Biological Chemistry , vol. 255 , pp. 10431-10438.
13.Frei, B., Kim, MC. and Ames, B. N., 1990,“Ubiquinol-10 is an effective lipid-soluble antioxidant at physiological concentrations”, Proc Natl Acad Sci , vol. 87 , pp. 4879-4883.
14.Faff, J. and Frankkiewicz-Jo zko ., 1997 ,“A effect of ubiquinone on exercise- induced lipid peroxidation in rat tissues”, European Journal of Applied Physiology , vol. 75 , pp. 413.
15.Gaber, B. P., Yager, P., Shriedan, J. P. and Chang, E. L., 1983 ,“Encapsulatiom of Hemoglobin in Phospholipid Vesicles”, FEBS Lett , vol. 153, pp. 285-288.
16.Hamilton, R. L., Luke, J. G., Guo, S. S., Williams, M. C. and Havel, R. L., 1980,“Unilamellar Liposomes made with the French Oressure Cell︰A Simple Preparation and Semiquantitative Technique”, Journal Lipid research , vol. 21 , pp. 981-992.
17.Hope, M. J., Bally, M. B., Mayer, L. D., Janoff, A. S. and Cullis, P.R., 1986 ,“Generation of multilamellar and umilamellar phospholipid vesicles”, Chemistry and Physics of Lipids , vol. 40 , pp. 89-107.
18.Junginger, H. E., Hofland, H. E. J. and Bouwstra, J. A., 1991,“Liposomes and niosomes:interactions with human skin”, Cosmetics & Toiletries Magazine , vol. 106 , pp. 45-50.
19.Kirby, C. and Gregoriadis, G., 1984 ,“Dehydration-rehydration vesicles: a simple method for high yield drug entrapment in liposomes”, Biotech , vol. 11 , pp. 979-984.
20.Kim, H. H. and Baianu, I. C., 1991,“Novie lipospme microencapsulation techniques for food applications”, Food Science & Technology , pp. 55-61
21.Komatsu, H. and Okada, S., 1995 ,“Ethanol-induced aggregation and fusion of small phosphatidylcholine liposome: participation of interdigitated membrane formation in their processes”, Biochim Biophys Acta , vol. 1235 , pp. 270.
22.Lasch, J., Laub, R. and Wohlrab, W., 1991,“How deep do intact liposomes penetrate into human skin?”, Journal of Controlled Release, vol. 18 , pp. 55-58.
23.Lasic, D.D., 1993,“Liposomes from physics to application”, Elseiver, New York , vol. 51 , pp. 213-220.
24.Mezei, M. and Gulasekharam, V., 1980,“Liposomes – a selective drug delivery system for the topical route of administration”, Life Sci , vol. 26 , pp. 1473-1477.
25.New, R. R. C., 1990 ,“In Liposome: a practical approach”, New York Chapter , vol. 1 , pp. 221-225.
26.Philippott, J., Mutaftschiev, S. and Liautard, J. P., 1976 ,“The preparation of large single bilayer liposomes by a fast and controlled dialysis”, Biochimica et Biophysica Acta , vol. 433 , pp. 629-634.
27.Schaller, M. and Korting, H. C., 1996 ,“Interaction of liposomes with human skin:the role of the stratum corneum”, Advanced Drug Delivery Reviews , vol. 18 , pp. 303-309.
28.Tagawa, M., Shinozaki, K., Kurata, Y., Matsumoto, K. and Tabata, Y., 1986 ,“Applications of hydrogenated lecithin for cosmetics”, 14th IFSCC Congress , Vol.1 , pp. 335.
29.Touitou, E., Alkabes, M., Dayan, N. and Eliaz, M., 1997,“Ethosomes: novel vesicular carriers for enhanced skin delivery”, Pharm Res , vol. 14 , pp. 305.
30.Ylikoski, T., Piirainen, J., Hanninen, O. and Penttinen, J., 1997,“The effect of coenzyme Q10 on the exercise performance of cross-country skiers”, Molecular Aspects of Medicine , vol. 18 , pp. 283-290.
31.Zuliani, U., Bonetti, A., Campana, M., Cerioli, G., Solito, F. and Novarini, A., 1989,“The influence of ubiquinone (Co Q10) on the metabolic response to work”, The Journal of Sports Medicine and Physical Fitness , vol. 29 , pp. 57-62.
32.Zellmer, W., Pfeil and Lasch, J.,1994 ,“Interaction of phosphatidylcholine liposomes with the human stratum corneum”, Biochim Biophys Acta ,vol. 1237 , pp. 176-182.
中文部份
33.方嘉良,2005,上班族女性臉部膚質的量化評估,嘉南藥理科技大學化妝品科技
研究所,碩士論文。
34.吳慧君,2002,“輔酶Q10與運動能力表現”,大專體育,58期,頁95~100,2 月
35.呂適任,2002,適於超音波藥物傳輸系統使用之微脂粒的製備與驗證,國立臺灣
大學電機工程學研究所,碩士論文。
36.林虔宏,2001,蛋黃卵磷脂微膠囊之製作及其物理性質之探討,國立中興大學畜
產產學系,碩士論文。
37.林朝欽,2005,微脂粒與膽鹽之間的交互作用,國立台灣科技大學化學工程系,碩士論文。
38.邱智信,2004,不同磷脂質包覆維生素E之微脂粒系統特性研究,南台科技大學
化學工程系研究所,碩士論文。
39.洪佳惠,2002膽固醇與膽鹽對微脂粒穩定度的影響,國立中央大學化學工程與材料工程研究所,碩士論文。
40.洪偉章,2001,化妝品有效性評估,高立圖書有限公司,台北縣。
41.徐珮清,2005,維生素C磷酸鎂微脂粒之製備及功能性研究,樹德科技大學應用設計研究所,碩士論文。
42.高佳萍,2004,不同磷脂質配方對於菸鹼胺皮膚穿之研究,國立清華大學化學工程研究所,碩士論文。
43.高登山,2001,微脂粒處方之修飾及其細胞轉染研究,國防大學國防醫學院生物化學研究所,碩士論文。
44.張志。1983,冷凍乾燥法,pp.7-12。徐氏基金會。
45.張季平,1991,“輔酶Q10在內科治療中之應用”,當代醫學,18卷,9期,頁 37~41,9月。
46.陳宜嫻,2000,皮膚黑色素抑制劑與保濕劑效能之研究,靜宜大學應用化學研究,碩士論文。
47.陳麗妃,2007,微脂粒包覆蠶絲蛋白之製備與功能性評估,樹德科技大學應用設
計研究所,碩士論文。
48.曾文良,2000,以離子型界劑溶解微脂粒之研究,國立中央大學化學工程研究所,碩士論文。
49.黃伯高,1998,”一種新的創面覆蓋物----絲素膜”,中華整型外科雜誌,14(4):270—274。
50.黃妍菱,2005,微脂粒包覆一系列茶多酚其物理化學性質及體內藥物動力學研究,長庚大學天然藥物研究所,碩士論文。
51.楊育才,2004,以微脂粒包覆光感物質之光動力效應探討,臺北醫學大學生物醫學材料研究所,碩士論文。
52.溫慧萍,1999,水溶性幾丁聚醣在含微脂粒活力滋潤霜的應用,國立台灣海洋大學食品科學系,碩士論文。
53.劉得任,1999,微卡計於液膜交互作用力量測之應用----微脂粒系統穩定度之探討,國立中央大學化學工程研究所,博士論文。
54.劉得任,2005,神奇之奈米微脂球,農業生技產業季刊,第二期。
55.劉鎧維,2000,膽固醇衍生物作為正電荷微脂粒組成之探討及改進基因載體微脂粒安定性之研究,國立臺灣大學藥學研究所,碩士論文。
56.鄭杏孚,2001,補充Co Q10對運動員體內抗氧化效力及運動表現之影響,輔仁大學食品營養學系,碩士論文。
57.謝宜芳,2000,不同脂質組合對微脂粒包覆率及安定性之影響,國立臺灣大學食品科技研究所,碩士論文。
58.謝憲明,2003,紫外光誘導光敏感微脂粒於藥物釋放之研究,暨南國際大學應用化學研究所,碩士論文。
59.羅浚育,1999,界面活性劑與微脂粒的作用,國立中央大學化學工程研究所,碩士論文。
60.蘇至善,2003,以批式超臨界反溶劑沉積法進行非類固醇抗發炎藥之再結晶研究,國立台灣大學化學工程學研究所,碩士論文。
61.蘇恆輝,1998,含胺基甲基葉酸微脂粒在膠原蛋白基材內之製備與應用,國立陽明大學醫學工程研究所,碩士論文。
62.鐘曉慧,2007,微脂粒包覆Co Q10之製備與特性評估研究,樹德科技大學應用設計研究所,碩士論文。

日文部份
63.佐佐木一郎與鈴木正,1992,“以磷脂質為中心的保溼劑之開發”,Flavour and Fragrance Journal,pp.22-28.
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
* *